Showing posts with label DNA Delivery to primary cells. Show all posts
Showing posts with label DNA Delivery to primary cells. Show all posts

Monday, August 3, 2015

Introducing Sleeping Beauty

New Technology for Gene Transfer From Delivery to Stable Expression
Neuromics has a successful track record of helping our clients delivery siRNA, miRNA, Plasmids and other oligos in vitro and in vivo with our Transfection Kits...But my vision with our cell based assay solutions has always been to provide engineered cells and plasmids modified to study your genes of interest. I am pleased to announce we are working with Smart Cell /B-MoGen Technologies to make this happen. We now can provide:
Gene Transfer and Expression Products Leveraging the Sleeping Beauty Technology

Images: B-MoGen Transposon exhibiting stable expression of five fluorescent genes
Advantages of Sleeping Beauty Transposon System:
· Delivery method is time and cost effective compared to lentiviral delivery.
· Increased cargo-capacity when compared to lentiviral delivery.
· Safest insertion profile of all gene transfer methods.
· Commonly integrated as a single copy.
Custom vector design and assembly, including multi-gene (up to 6) vectors.
We are in the process of formulating standard offerings. In the meantime, I am positioned to offer favorable pricing and terms to early adopters of our Sleeping Beauty Solutions. Please contact me directly pshuster@neuromics.com or 612-801-1007. We can together determine your needs and desired outcomes and provide a statement of work with pricing, project milestones and delivery.

Thursday, December 15, 2011

Delivering DNA to Cell Lines and Primary Cells

Neuromics is pleased to introduce DNA-FectTM and DNA-FectTM293 in vivo transfection reagents.

These reagents deliver genes to various established cell lines as well as primary cells, which include HEK293, 293T, 293E, CHO, COS1, HeLa, NIH 3T3, insect cell lines (Sf9 and Sf21) and a variety of other eucaryotic cell lines with low cytotoxicity. GeneExpresso™ MAX reagent, 1.0 ml, is sufficient for 300 to 600 transfections in 24 well plates, or 150 to 300 transfections in 6 well plates.


Capabilities include:
• Proven to deliver DNA to difficult-to-transfect cells
•Stable and easy to use
•Suitable for high-thoroughput (HTS) applications

Image: Comparing DNA-Fect vs Lipofectamine 2000 by FACS Analysis





DNA Delivery Protocol: