Showing posts with label DNA transfection. Show all posts
Showing posts with label DNA transfection. Show all posts

Wednesday, January 20, 2016

Delivery of siRNA, miRNA, shRNA and Plasmids Guaranteed

Potent and Frequently Published Transfection Solutions

Gene Tools
We have proven and frequently published transfection solutions
Powerful punch and versatility are now needed more than ever with the 
hyper growth in gene manipulation technologies like Sleeping BeautyTM
and CRISPR-Cas9.

We have published examples of the use of our solutions for delivering siRNA, miRNA, 

shRNA and plasmids both in vitro and in vivo. here are some of your options.
i-Fect ™ -A novel cationic lipid formulation specifically 
designed for efficient delivery of 27mer DsiRNAs(dicer 
substrate small Interfering RNAs)& 21mer siRNAs (small interfering RNAs) in vitro and in vivo.
p-Fect™ -Designed to delivery plasmids, DNA or 
RNA to hard to transfect Cell Lines.
pn-Fect™ -The latest advance in transfection 
for primary neuronal cells. 
This unique reagent provides ultra-high plasmid DNA delivery 
efficiencies and low cytotoxicity compared to competitive reagents.
Here's a recent i-Fect Publication:
Liuming Jiang, Qun Wu , Tao Yang. Silencing of Id2 Alleviates 
Chronic Neuropathic Pain Following Chronic Constriction Injury.
Journal of Molecular Neuroscience\pp 1-7.First online: 15 January 2016
 i-Fect
Figure: Knockdown of Id2 attenuated mechanical allodynia and thermal 
hyperalgesia in CCI rats. (a and b) PWT and PWL were measured 1 day before 
CCI and 1, 3, 7, and 14 days after intrathecal administration of shRNA-Id2.
If you are looking for transfection solutions, do not hesitate to contact me @ direct phone: 612-801-1007 or pshuster@neuromics.com. Thank you, Pete Shuster, CEO and Owner, Neuromics

Thursday, December 15, 2011

Delivering DNA to Cell Lines and Primary Cells

Neuromics is pleased to introduce DNA-FectTM and DNA-FectTM293 in vivo transfection reagents.

These reagents deliver genes to various established cell lines as well as primary cells, which include HEK293, 293T, 293E, CHO, COS1, HeLa, NIH 3T3, insect cell lines (Sf9 and Sf21) and a variety of other eucaryotic cell lines with low cytotoxicity. GeneExpresso™ MAX reagent, 1.0 ml, is sufficient for 300 to 600 transfections in 24 well plates, or 150 to 300 transfections in 6 well plates.


Capabilities include:
• Proven to deliver DNA to difficult-to-transfect cells
•Stable and easy to use
•Suitable for high-thoroughput (HTS) applications

Image: Comparing DNA-Fect vs Lipofectamine 2000 by FACS Analysis





DNA Delivery Protocol: