Protocols for Delivering siRNA to Neurons

Here's a link to the protocol: Transfecting Schwann Cells with i-Fect and below are related data.

Figure:si RNA-mediated suppression of target gene expression in Schwann cells. A, Detergent extracts of siNeg- or siGly1- transfected Schwann cells were digested with heparitinase and subjected to immunoblot analysis with anti-glypican-1 antibodies (top);aliquots of undigested extracts wereimmunoblotted with anti-actin antibodies (bottom) to verify equal sample loading. B, Cell surface expression of glypican-1 was assessed by immunofluorescent staining of transfected cells 48 h after transfection using anti-glypican- 1 antibodies (green) and DAPI (4',6'-diamidino-2 phenylindole) to stain nuclei (red). C, Schwann cells were transfected with siNeg or si 4(V)and conditioned media and cell lysates were harvested 48 h later (left) or at the indicated times after transfection (right);aliquots of medium (top) or cell lysates (bottom) were subjected to immunoblot analysis and stained with anti- 4(V) collagen (top) or anti- -actin (b ottom) antibodies.

intrathecal in vivo delivery of siRNA(pdf - 57Kb)

Publications:

Progress Towards in vivo Use of siRNAs
siRNAi: Applications in Functional Genomics and Potential as Therapeutics
Assembly and Function of RNAi Silencing Complexes
Synthetic dsRNA Substrates Enhance RNAi Potency and Efficacy