Friday, June 25, 2010

siRNA and i-Fect for the Study of Retinal Disease

We continue to add new references to the many ways researchers are using i-FectTM to increase the potency of siRNA delivery.

This is a new reference from the book entitled Retinal Degenerative Diseases: Laboratory and Therapeutic Investigations By Robert E. Anderson, Joe G. Hollyfield, Matthew M. Lavail.

In this study, researchers used i-Fect to transfect and immortalized cell line from Mouse cones (661W) expressing ELOVL4. Using siRNA designed to silence the ELOVL4 gene, they used i-Fect + siRNA to transfect cells cultured at a density of 2X105. Knockdown was achieved as confirmed by western blot analysis.

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