Monday, July 29, 2013

i-Fect siRNA Transfection Successes

Meeting your Gene Expression Analysis Requirements.

I have posted many success stories resulting from use of  Neuromics'  Neuromics' i-FectTM siRNA Transfection Kit. It has been 3 months since my last posting so here I would like to post some recent highlights.

Here I feature customer publication demonstrating:
  1. Down regulation of Neuroligin 2-data showed unexpected upregulation and pronociceptive effects of the “inhibitory” NL2 in neuropathic pain, suggesting a functional shift of NL2 from inhibition to excitation that changed the synaptic ratio toward higher excitation.
  2. Role of IGFPB5 for survival of neurons in post-natal development.
  3. HDAC role in Anxiety and Alcoholsim
Here're the publications:
Tiphaine Dolique, Alexandre Favereaux, Olivier Roca-Lapirot, Virginie Roques, Claire Léger, Marc Landy, Frédéric Nagy, Unexpected association of the “inhibitory” Neuroligin 2 with excitatory PSD95 in neuropathic pain, PAIN, Available online 25 July 2013, ISSN 0304-3959,
( siRNAs were solubilized in 10 µl of i-Fect reagent (Neuromics, Edina, Minnesota, USA) following Neuromics instructions and published protocol, and applied intrathecally using a Hamilton syringe...

Masaki Ueno,Yuki Fujita, Tatsuhide Tanaka, Yuka Nakamura, Junichi Kikuta, Masaru Ishii & Toshihide Yamashita. Layer V cortical neurons require microglial support for survival during postnatal development. Nature Neuroscience 16, 543–551 (2013) doi:10.1038/nn.3358.
...vehicle (PBS) was delivered intraventricularly through the cisterna magna with a glass pipette while P3 mice were cold anesthetized. Igf1 siRNA (stealth siRNA, Invitrogen) or Igfbp5 siRNA with i-Fect reagent (Neuromics) was delivered intraventricularly through the cisterna magna at P3 twice with a 12-h interval...

Sachin Moonat, Amul J. Sakharkar, Huaibo Zhang, Lei Tanga, Subhash C. Pandey. Aberrant Histone Deacetylase2–Mediated Histone Modifications and Synaptic Plasticity in the Amygdala Predisposes to Anxiety and Alcoholism. Biological Psychiatry.
...The siRNAs were dissolved in iFect solution (Neuromics, Edina)...

I will keep updating you as more pubs and data becomes available.

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